Alpha-interferon inhibits the expression of heavy chain mu messenger RNA in Daudi cells.

摘要: Abstract A clone of Daudi cells (Daudi-S) synthesizes the heavy chain IgM (mu-chain) under routine conditions cell culture. In presence alpha-interferon, however, synthesis mu-chain is decreased rapidly at a time when overall protein not modified and dsRNA-dependent kinase 2-5A synthetase are induced. This inhibition seems to be correlated with antiproliferative action interferon since it occurs only slightly in another resistant (Daudi-R) interferon. these cells, induced by Specific interferon-treated Daudi-S consequence steady-state levels mRNA. effect 4-8 h after addition parallel c-myc mRNA enhanced HLA Reduced its degradation as shown actinomycin D chase experiments. Furthermore, nuclear run on experiments rule out an transcription Therefore, inhibitory mechanism mediated might level termination and/or post-transcriptional processing RNA. contrast, same gene expression due (in accord other reports). These data indicate that can inhibit different mechanisms.