Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei

摘要: Ferritin is traditionally considered a cytoplasmic iron-storage protein, but recent reports indicate that it also found in cell nuclei. Nuclear ferritin has been proposed to be involved both the protection of DNA and exacerbation iron-induced oxidative damage DNA. We demonstrate H-rich present nucleus human astrocytoma tumor cells. To study mechanism regulation translocation nucleus, we developed culture model using SW1088 Changes cellular iron levels, cytokine treatments hydrogen peroxide exposure affected distribution between cytosol nucleus. enters via active transport through nuclear pore does not require NLS-bearing cytosolic factors for transport. Furthermore, preferred over L-rich uptake into Whole crosslinking studies revealed associated with protected from vitro models. These results strongly suggest novel role protection. This work should lead characterization functions context genomic stability may have unparalleled biological significance terms accessibility metals The knowledge generated as result these will improve our understanding constituents.