Novel oligo(ethylene glycol)-based molecularly imprinted magnetic nanoparticles for thermally modulated capture and release of lysozyme.

作者: Nan Li , Li Qi , Ying Shen , Juan Qiao , Yi Chen

DOI: 10.1021/AM505427J

关键词:

摘要: In this study, oligo(ethylene glycol) (OEG)-based thermoresponsive molecularly imprinted polymers (MIPs) for lysozyme on the surface of magnetic nanoparticles were synthesized. Thermoresponsive monomer 2-(2-methoxyethoxy)ethyl methacrylate, chelate N-(4-vinyl)-benzyl iminodiacetic acid, and acidic methacrylic acid selected as ingredients preparing MIP layer. The behavior novel was evaluated by dynamic light scattering swelling ratios measurements. Interestingly, in analysis lysozyme, capture/release process could be modulated changing temperature, avoiding tedious washing steps. Meanwhile, high adsorption capacity (204.1 mg/g) good selectivity capturing achieved. Additionally, imprinting with substrate allowed short time (2 h) rapid separation. Furthermore, proposed used to s...

参考文章(32)
Ying Shen, Wei Guo, Li Qi, Juan Qiao, Fuyi Wang, Lanqun Mao, Immobilization of trypsin via reactive polymer grafting from magnetic nanoparticles for microwave-assisted digestion Journal of Materials Chemistry B. ,vol. 1, pp. 2260- 2267 ,(2013) , 10.1039/C3TB20116C
Zian Lin, Zhiwei Xia, Jiangnan Zheng, Duo Zheng, Lan Zhang, Huanghao Yang, Guonan Chen, Synthesis of uniformly sized molecularly imprinted polymer-coated silica nanoparticles for selective recognition and enrichment of lysozyme Journal of Materials Chemistry. ,vol. 22, pp. 17914- 17922 ,(2012) , 10.1039/C2JM32734A
Lei Qin, Xi-Wen He, Wei Zhang, Wen-You Li, Yu-Kui Zhang, Macroporous thermosensitive imprinted hydrogel for recognition of protein by metal coordinate interaction. Analytical Chemistry. ,vol. 81, pp. 7206- 7216 ,(2009) , 10.1021/AC900676T
Huachang Chen, Juan Kong, Dongying Yuan, Guoqi Fu, Synthesis of surface molecularly imprinted nanoparticles for recognition of lysozyme using a metal coordination monomer. Biosensors and Bioelectronics. ,vol. 53, pp. 5- 11 ,(2014) , 10.1016/J.BIOS.2013.09.037
A. Bossi, F. Bonini, A.P.F. Turner, S.A. Piletsky, Molecularly imprinted polymers for the recognition of proteins: The state of the art Biosensors and Bioelectronics. ,vol. 22, pp. 1131- 1137 ,(2007) , 10.1016/J.BIOS.2006.06.023
Mehmet Odabaşi, Ridvan Say, Adil Denizli, Molecular imprinted particles for lysozyme purification Materials Science and Engineering: C. ,vol. 27, pp. 90- 99 ,(2007) , 10.1016/J.MSEC.2006.03.002
K. Aguilar-Arteaga, J.A. Rodriguez, E. Barrado, Magnetic solids in analytical chemistry: A review Analytica Chimica Acta. ,vol. 674, pp. 157- 165 ,(2010) , 10.1016/J.ACA.2010.06.043
Tao Jing, Hairong Du, Qing Dai, Huan Xia, Jiwei Niu, Qiaolin Hao, Surong Mei, Yikai Zhou, Magnetic molecularly imprinted nanoparticles for recognition of lysozyme Biosensors and Bioelectronics. ,vol. 26, pp. 301- 306 ,(2010) , 10.1016/J.BIOS.2010.08.044
Guoqing Pan, Qianping Guo, Chengbin Cao, Huilin Yang, Bin Li, Thermo-responsive molecularly imprinted nanogels for specific recognition and controlled release of proteins Soft Matter. ,vol. 9, pp. 3840- 3850 ,(2013) , 10.1039/C3SM27505A