Effects of corilagin on alleviating cholestasis via farnesoid X receptor-associated pathways in vitro and in vivo.

摘要: BACKGROUND AND PURPOSE The aim of this study was to investigate the ameliorative effects corilagin on intrahepatic cholestasis induced by regulating liver farnesoid X receptor (FXR)-associated pathways in vitro and vivo. EXPERIMENTAL APPROACH Cellular animal models were treated with different concentrations corilagin. In cellular experiments, FXR expression up-regulated either lentiviral transduction or GW4064 treatment down-regulated siRNA technology guggulsterones. Real-time PCR Western blotting employed detect mRNA protein levels FXR, SHP1, SHP2, UGT2B4, BSEP, CYP7A1, CYP7B1, NTCP, MRP2 SULT2A1. Immunohistochemistry used examine BSEP tissues. Rat function pathological changes hepatic tissue assessed using biochemical tests haematoxylin eosin staining. RESULTS Corilagin increased SULT2A1, decreased those CYP7B1 NTCP. After up- down-regulating methods, could still increase SULT2A1 decrease especially when administered at a high concentration. also exerted notable effect manifestations cholestasis, staining tissues function. CONCLUSIONS IMPLICATIONS exerts protective hepatocytes can prevent deleterious activities stimulating FXR-associated pathways.