Quantitative reverse transcription polymerase chain reaction-based detection of thyroid-specific gene expression in fine-needle aspirate for thyroid cancer recurrence evaluation: a case report and review of the literature.

摘要: Background Despite improved surveillance for patients after total thyroidectomy cancer, there has yet to be a diagnostic method that detects recurrence with 100% accuracy. Methods A 60-year-old woman family history of papillary thyroid cancer (PTC) underwent and radioactive iodine ablation. On postoperative surveillance, ultrasound examination the neck demonstrated focus concerning fine-needle aspirate (FNA) was performed. The cytology report nondiagnostic and, hence, RNA extracted from specimen followed by reverse transcription (cDNA), quantitative real-time polymerase chain reaction (qRT-PCR) detect thyroid-specific gene expression (thyroglobulin =Tg; sodium-iodide symporter = NIS; thyroperoxidase = TPO). Results Expression select genes demonstrated, given patient's remarkable history, surgical resection elected. Final pathology follicular adenoma. Conclusion This case demonstrates novel approach used in evaluation recurrent as an adjunct FNA cytology. © 2015 Wiley Periodicals, Inc. Head Neck 37: E165–E168,