Collagen microgel-assisted dexamethasone release from PLLA-collagen hybrid scaffolds of controlled pore structure for osteogenic differentiation of mesenchymal stem cells.
作者： Himansu Sekhar Nanda , Tomoko Nakamoto , Shangwu Chen , Rong Cai , Naoki Kawazoe
DOI: 10.1080/09205063.2014.938980
关键词:
摘要: Directed stem cell differentiation over three-dimensional porous scaffolds capable of releasing bioactive instructive cues is an important tool in tissue engineering. In this research, we have prepared dexamethasone (Dex)-releasing collagen microbead-functionalized poly(L-Lactide)-collagen hybrid as osteoinductive platform for human bone marrow-derived mesenchymal cells (MSCs). The were by a combined method emulsion freeze-drying and porogen-leaching using pre-prepared ice particulates porogen material. Dex release from the was studied at 37 °C under shaking condition impact released towards osteogenic lineage investigated 3 week vitro culture MSCs. results showed that had controlled pore structure interconnected pores deposited with fibers. scaffold facilitated seeding spatial localization Dex/collagen microbeads micr...
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