Genome-wide functional profiling reveals genes required for tolerance to benzene metabolites in yeast.
作者： Matthew North , Vickram J. Tandon , Reuben Thomas , Alex Loguinov , Inna Gerlovina
DOI: 10.1371/JOURNAL.PONE.0024205
关键词:
摘要: Benzene is a ubiquitous environmental contaminant and widely used in industry. Exposure to benzene causes number of serious health problems, including blood disorders leukemia. undergoes complex metabolism humans, making mechanistic determination toxicity difficult. We functional genomics approach identify the genes that modulate cellular three phenolic metabolites benzene, hydroquinone (HQ), catechol (CAT) 1,2,4-benzenetriol (BT), model eukaryote Saccharomyces cerevisiae. generate oxidative cytoskeletal stress, tolerance requires correct regulation iron homeostasis vacuolar ATPase. have identified conserved bZIP transcription factor, Yap3p, as important for HQ-specific response pathway, well two encode putative NAD(P)H:quinone oxidoreductases, PST2 YCP4. Many yeast human orthologs may similar manner could play role exposure-related disease.
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