Identification of genes with abnormal expression changes in acute myeloid leukemia.

摘要: Acute myeloid leukemia (AML) is one of the most common and deadly forms hematopoietic malignancies. We hypothesized that microarray studies could identify previously unrecognized expression changes occur only in AML blasts. were particularly interested those genes with increased AML, believing these may be potential therapeutic targets. To test this hypothesis, we compared gene profiles between normal cells from 38 healthy donors leukemic blasts 26 patients. Normal samples included CD34+ selected (N = 18), unselected bone marrows 10), peripheral bloods 10). Twenty displayed AML-specific not found cells. Subsequent analyses using data 285 additional patients confirmed for 13 20 genes. Seven (BIK, CCNA1, FUT4, IL3RA, HOMER3, JAG1, WT1) while 6 (ALDHA1A, PELO, PLXNC1, PRUNE, SERPINB9, TRIB2) decreased expression. Quantitative RT/PCR 7 over-expressed performed an independent set 9 21 pediatric samples. All to normals. Three (WT1, IL3RA) have already been linked leukemogenesis and/or prognosis, little known about role other 4 AML. Future will determine their clinical significance.