Apoptosis and gene expression of collagenases but not gelatinases in rabbit disc fragment cultures.

摘要: Object. The object of this study was to characterize the biological response isolated intervertebral disc fragments in vitro culture conditions with respect cell death and inflammatory catabolic changes. acquired data could help gain a better understanding reaction tissue when exposed environmental changes along altered nutritional osmotic conditions, as are encountered different models or diseases vivo. Methods. Intervertebral anulus were from Burgundy rabbits cultured standard media for 3 days. analyzed their swelling properties, proteoglycan loss on histological studies, lactate dehydrogenase activity, apoptosis, gene expression collagenases gelatinases, proinflammatory (MCP-1, IL-8, IL-6) apoptosis-associated (TNF-a, Fas-L, caspase 3) genes. Results. results demonstrate that specimens swelling, proteoglycans disarrangement architecture observed. cells underwent rapid apoptosis upregulation various Both collagenases, matrix metalloproteinase (MMP)‐1 MMP-13, increasingly transcribed, whereas gelatinases MMP-2 MMP-9 did not respond downregulated. Conclusions. Cultured swell undergo necrotic apoptotic combined chemoattractant proteins. Some these findings have been demonstrated before spinal disorders. In addition, suitable long-term if stable metabolism is desired, described be considered using material cultures. (DOI: 10.3171/SPI/2008/8/6/552)