Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response
作者： Cherukuri Sudhakar , Lakshmi N Mangamoori
DOI: 10.1111/J.1440-1746.2007.05012.X
关键词:
摘要: Background and Aim: Rat Zajdela ascitic hepatocellular carcinoma (ZAH) is a malignant cell type with some properties in common rat hepatocytes. The aim of this study was to determine the glycosylation patterns surface glycoproteins two lines C D ZAH by lectin binding for identification characterization tumor-specific markers. Methods: Plasma membrane proteins from these cells were separated polyacrylamide gel electrophoresis presence sodium dodecyl sulfate, probed using radio-iodinated lectins. Results: We observed decrease concavalin A, an increase wheat germ agglutinin (WGA) tumor as compared those normal liver cells. showed that WGA mainly due increased sialylation major sialylated contained O-linked carbohydrate chains. It also shown removal sialic acid neuraminidase significantly decreased lethality led survival tumor-bearing animals. appears be antigenicity desialylated cells. Conclusion: Taken together, highly gp120 its absence significance respect biological tumor.
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