A non-alkalophilic mutant of Bacillusalcalophilus lacks the Na+H+ antiporter

作者: Terry A. Krulwich , Kenneth G. Mandel , Robert F. Bornstein , Arthur A. Guffanti

DOI: 10.1016/0006-291X(79)90582-5

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摘要: A non-alkalophilic mutant strain of Bacillusalcalophilus grows on L-malate over a pH range from 5.0 to 9.0. The does not exhibit the energy-dependent efflux Na+ that has been used assay Na+H+ antiporter in wild type organism. also fails transport α-aminoisobutyric acid, at 9.0, either presence or absence Na+; 5.5, amino acid analogue is taken up by Na+-independent mechanism. properties constitute strong evidence involved maintaining an acidified cytoplasm B. alcalophilus.

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