Optimized assay for serum angiotensin-converting enzyme activity.

摘要: This optimized two-point kinetic assay for serum angiotensin-converting enzyme is based on the colorimetric determination of hippurate with cyanuric chloride/dioxan reagent. The released from hippuryl-L-histidyl-L-leucine by in presence chloride ion. CVs method (within-run and between-run) ranged 2.1 to 3.2%. Linearity extends up 200 U/L. Results are unaffected lipemia icterus. Hemoglobin concentrations greater than 1.5 g/L causes a slight negative interference. Reference intervals men women 22-82 U/L 25-69 U/L, respectively.