Live-cell reporters for fluorescence imaging.
作者： Ivan R Corrêa Jr
DOI: 10.1016/J.CBPA.2014.04.007
关键词:
摘要: Advances in the development of new fluorescent reporters and imaging techniques have revolutionized our ability to directly visualize biological processes living systems. Real-time analysis protein localization, dynamics, interactions has been made possible by site-specific labeling with custom designed probes. This review outlines some most recent advances design application live-cell probes, a particular focus on SNAP-tag technology. Specific examples illustrating applications superresolution single-molecule imaging, trafficking recycling, protein-protein are presented.
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