Molecular docking and inhibition of matrix metalloproteinase-2 by novel difluorinatedbenzylidene curcumin analog
作者： Fazlul H Sarkar , Subhash Padhye , Anita Suri , Vivek Sharma , Arundhati Saraph
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摘要: We recently described the synthesis and characterization of a novel difluorinatedbenzylidene analog curcumin, commonly referred as CDF, which demonstrated significantly enhanced bioavailability in vivo anticancer activity. CDF targets many factors similar to albeit with more potency, reported previously. To further highlight this differential behavior we chose matrix metalloproteinase protein MMP-2 is involved processes invasion metastasis human tumors. Both curcumin were characterized for their binding characteristics using silico docking studies; they also evaluated via biological assays involving gelatin zymography, miRNA analysis, ELISA. was found inhibit expression activity A549 H1299 NSCLC cells much effectively than validating molecular modeling results. miR-874, an MMP-2-targeting miRNA, up-regulated by CDF. Thus, it appears that can through multiple mechanisms. Our results are suggestive potent inhibition compared thus warranting its evaluation effective agent.
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