Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties

摘要: Biocatalysis presents a sound alternative to chemical synthesis in the field of drug production, given highly selective nature biological catalysts. Penicillin G Acylase (PGA) from E. coli is currently used hydrolyze penicillin (PG) and catalyzes β-lactam antibiotics. In this work, particular emphasis recent developments acylase immobilization, by entrapment simultaneously with nano-magnetic particles silica matrix. The sol–gel biocatalytic were prepared either conventional method (crushed powder) or more approach, based an emulsion system using 150 mM AOT/isooctane, which allowed for formation spherical micro- nanobeads. effects on PGA activity different precursors, additives, enzyme concentration, aging, drying conditions mechanical stability evaluated. After these optimization studies, mechanically stable carrier porous xerogels matrixes, starting tetramethoxysilane (TMOS) 65–67% yield carriers immobilization 74 mg protein gdry sol–gel−1 930 Ugdry specific obtained.