Detoxification-related gene expression accompanies anhydrobiosis in the foliar nematode (Aphelenchoides fragariae).

摘要: The foliar nematode (Aphelenchoides fragariae) is a quarantined pest that infects broad range of herbaceous and woody plants. Previous work has demonstrated its remarkable ability to survive rapid extreme desiccation, although the specific molecular mechanisms underlying anhydrobiotic response have not been characterized. authors used RNA sequencing de novo transcriptome assembly compare patterns gene expression between hydrated 24-hr desiccated nematodes. In total, 2,083 953 genes were significantly up- downregulated, respectively, in Of 100 annotated with largest positive fold-changes, more than one third encoded putative detoxification-related proteins. Genes encoding enzymes Phase I II detoxification systems among most strongly upregulated transcriptome, including 35 cytochrome p450s, 23 short chain dehydrogenase/reductases, 5 glutathione-S-transferases, 22 UDP-glucuronosyltransferases. heat shock proteins, unfolded protein enzymes, intrinsically disordered proteins also upregulated. Anhydrobiosis A. fragariae appears involve both strategies minimize misfolding aggregation, wholesale induction cellular machinery. These processes may be controlled part through activity forkhead transcription factors similar Caenorhabditis elegans' daf-16, number which differentially expressed under desiccation. elegans’