Transcription Regulation of ompF and ompC by a Single Transcription Factor, OmpR
作者： Takeshi Yoshida , Ling Qin , Linda A. Egger , Masayori Inouye
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摘要: The ompF and ompC genes of Escherichia coli are reciprocally regulated by a single transcription factor, phosphorylated OmpR (OmpR-P), depending upon medium osmolarity. This regulation involves activation its repression with concomitant ompC. occurs through OmpR-P binding to four (F1, F2, F3, F4) three (C1, C2, C3) sites located upstream the promoters, respectively, novel mechanism. Here we show that there is distinct hierarchy within F1, F3 as well C1, C3 sites. Each these contains two tandem 10-bp OmpR-P-binding subsites, a-site b-site (from 5' 3' direction). has higher affinity downstream than in each case. Six molecules bind F C two-by-two discontinuous "galloping" manner. We propose this tight hierarchical OmpR, allows stepwise transcription, which minimizes their overlapping expression changes osmolarity achieve reciprocal
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