Apoptotic signaling induced by benzamide riboside: an in vitro study.
作者： Sujata Pathak , Chandresh Sharma , H. N. Jayaram , Neeta Singh
DOI: 10.1007/S11010-009-0075-8
关键词:
摘要: Benzamide riboside (BR) is a novel anticancer agent exhibiting potent cytotoxic activity in malignant cell lines. However, the mechanism of induction apoptosis not clear. The purpose this study was to elucidate apoptotic signaling induced by BR on different human cancer Our results revealed that at dose 50 μM induces SiHa, Hep2, and Ca Ski cells as studied morphology flow cytometry. A downregulation anti-apoptotic proteins Bcl-2 Bcl-xL observed, whereas expression level pro-apoptotic protein Bax remained unaffected. An upregulation p53 observed while no change seen inducing factor (AIF). significant increase caspase-3 -9 activities seen, which accompanied PARP cleavage. Release cytochrome c from mitochondria cytosol also observed. Taken together, findings suggest via intrinsic mitochondrial pathway.
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sci-hub.se 参考文章(23)
Michael Grusch, Georg Rosenberger, Gerhard Fuhrmann, Katja Braun, Birgit Titscher, Thomas Szekeres, Monika Fritzer-Skekeres, Georg Oberhuber, Karsten Krohn, Markus Hengstschlaeger, Georg Krupitza, Hiremagalur N Jayaram, Benzamide riboside induces apoptosis independent of Cdc25A expression in human ovarian carcinoma N.1 cells. Cell Death & Differentiation. ,vol. 6, pp. 736- 744 ,(1999) , 10.1038/SJ.CDD.4400546
Peter Rauko, Ladislav Novotny, Ima Dovinova, Luba Hunakova, Thomas Szekeres, Hiremagalur N. Jayaram, Antitumor activity of benzamide riboside and its combination with cisplatin and staurosporine. European Journal of Pharmaceutical Sciences. ,vol. 12, pp. 387- 394 ,(2001) , 10.1016/S0928-0987(00)00180-9
Ladislav Novotny, Peter Rauko, Joel Yalowitz, Thomas Szekeres, Antitumor activity of benzamide riboside in vitro and in vivo. Current Medicinal Chemistry. ,vol. 9, pp. 773- 779 ,(2002) , 10.2174/0929867024606894
Christoph Borner, The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions Molecular Immunology. ,vol. 39, pp. 615- 647 ,(2003) , 10.1016/S0161-5890(02)00252-3
A. Ashkenazi, Death Receptors: Signaling and Modulation Science. ,vol. 281, pp. 1305- 1308 ,(1998) , 10.1126/SCIENCE.281.5381.1305
Neeru Khanna, H.N Jayaram, Neeta Singh, Benzamide riboside induced mitochondrial mediated apoptosis in human lung cancer H520 cells. Life Sciences. ,vol. 75, pp. 179- 190 ,(2004) , 10.1016/J.LFS.2003.11.026
Himani Sharma, Sudip Sen, Neeta Singh, Molecular pathways in the chemosensitization of cisplatin by quercetin in human head and neck cancer. Cancer Biology & Therapy. ,vol. 4, pp. 949- 955 ,(2005) , 10.4161/CBT.4.9.1908
Chuan Ji, Ventkataraman Amarnath, Jennifer A Pietenpol, Lawrence J Marnett, None, 4-Hydroxynonenal Induces Apoptosis via Caspase-3 Activation and Cytochrome c Release Chemical Research in Toxicology. ,vol. 14, pp. 1090- 1096 ,(2001) , 10.1021/TX000186F
Joel Yalowitz, Hiremagalur Jayaram, Modulation of cytotoxicity of benzamide riboside by expression of NMN adenylyltransferase. Current Medicinal Chemistry. ,vol. 9, pp. 749- 758 ,(2002) , 10.2174/0929867024606867
W.G Tatton, C.W Olanow, Apoptosis in neurodegenerative diseases: the role of mitochondria. Biochimica et Biophysica Acta. ,vol. 1410, pp. 195- 213 ,(1999) , 10.1016/S0005-2728(98)00167-4