High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos.

作者: Adrianna Ianora , Angelo Fontana , Maria Costantini , Nadia Ruocco , Susan Costantini

DOI: 10.1371/JOURNAL.PONE.0172171

关键词:

摘要: … high-quality and large quantities of RNA from the copepod Calanus helgolandicus. … Qiagen RNeasy Micro Kit were the optimal isolation method for high-quality and quantity of RNA …

参考文章(43)
F. Zito, C. Costa, S. Sciarrino, C. Cavalcante, V. Poma, V. Matranga, Cell Adhesion and Communication: A Lesson from Echinoderm Embryos for the Exploitation of New Therapeutic Tools Progress in molecular and subcellular biology. ,vol. 39, pp. 7- 44 ,(2005) , 10.1007/3-540-27683-1_2
John M. Lawrence, Sea urchins : biology and ecology Elsevier. ,(2013)
Charles F. Boudouresque, Marc Verlaque, Ecology of Paracentrotus lividus Developments in Aquaculture and Fisheries Science. ,vol. 32, pp. 177- 216 ,(2001) , 10.1016/S0167-9309(01)80013-2
PHILIPPE GAYRAL, LUCY WEINERT, YLENIA CHIARI, GEORGIA TSAGKOGEORGA, MARION BALLENGHIEN, NICOLAS GALTIER, Next-generation sequencing of transcriptomes: a guide to RNA isolation in nonmodel animals. Molecular Ecology Resources. ,vol. 11, pp. 650- 661 ,(2011) , 10.1111/J.1755-0998.2011.03010.X
A. Miralto, G. Barone, G. Romano, S. A. Poulet, A. Ianora, G. L. Russo, I. Buttino, G. Mazzarella, M. Laabir, M. Cabrini, M. G. Giacobbe, The insidious effect of diatoms on copepod reproduction Nature. ,vol. 402, pp. 173- 176 ,(1999) , 10.1038/46023
Andreas Schroeder, Odilo Mueller, Susanne Stocker, Ruediger Salowsky, Michael Leiber, Marcus Gassmann, Samar Lightfoot, Wolfram Menzel, Martin Granzow, Thomas Ragg, The RIN: an RNA integrity number for assigning integrity values to RNA measurements BMC Molecular Biology. ,vol. 7, pp. 3- 3 ,(2006) , 10.1186/1471-2199-7-3
ANA RIESGO, ALICIA R. PÉREZ‐PORRO, SUSANA CARMONA, SALLY P. LEYS, GONZALO GIRIBET, Optimization of preservation and storage time of sponge tissues to obtain quality mRNA for next-generation sequencing. Molecular Ecology Resources. ,vol. 12, pp. 312- 322 ,(2012) , 10.1111/J.1755-0998.2011.03097.X
Annalisa Pinsino, Valeria Matranga, Francesca Trinchella, Maria Carmela Roccheri, Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects Ecotoxicology. ,vol. 19, pp. 555- 562 ,(2010) , 10.1007/S10646-009-0432-0
S. Dupont, O. Ortega-Martínez, M. Thorndyke, Impact of near-future ocean acidification on echinoderms. Ecotoxicology. ,vol. 19, pp. 449- 462 ,(2010) , 10.1007/S10646-010-0463-6