Production and characterization of human antirenin antibodies prepared with synthetic immunogens

摘要: Specific blockade of renin angiotensin system can be obtained both by enzymatic inhibitors and passive active immunization against renin. Recent studies have shown that synthetic peptides mimicking a protein segment used as immunogens to elicit antibodies which react with the parent molecule. In order develop antirenin antigens we selected peptidic sequences from human synthesized corresponding produce able recognize entire molecule inhibit its activity. Three dimensional models were predict 17 putative epitopes. Then 18 related 13 potential antigenic determinants solid or liquid phase technic 11 when tested their binding several polyclonal monoclonal antibodies. The injected into rabbits antisera radio-immunoassay (RIA) enzyme linked immunosorbent assay (ELISA) showed nine immunogenic. Mainly, antiserum raised peptide beta-hairpin 81-50 lies across catalytic cleft, produced 25 p. 100 inhibition plasma activity at 1: 50 dilution. Immunoglobulins, purified this epitopes, bound labelled inhibited pure on tetradecapeptide substrate, in dose dependent manner.