Fluorescence: a diagnostic tool for the detection of stress in plants

摘要: Green vegetation when excited by specific wavelengths of light dissipates a portion the absorbed energy as emissions in form fluorescence several broad areas spectrum. Currently, leaf level fluoresence have been broken down into five primary regions, namely; ultraviolet (UV), blue, green, red, and near-infrared (NIR). The optimal excitation for each these bands was verified healthy soybean leaves through use EEM. Intact at 280 nm emits substantial two bands; first centered near 335 nm, second 440 nm. UV band from treated with varying levels nitrogen decreases relative to blur function total protein concentration. These studies indicate that vivo can be utilized non-destructive tool remotely sense variations concentration due fertilization level. It has well established this emission originates proteins contain aromatic amino acids. majority plant acids result potential region spectrum discussed here. Pure ribulose 1,5-bisphosphate carboxylase aqueous solution exhibited intense characteristics distributions similar those intact vegetation. Due its high we believe contributes emanating leaf. red NIR within wavelength 250 675 maxima 430 470 600 660 ratio spectra produces which exhibits striking similarities action photosynthesis. differences between depend on excitation. Moreover, comparing versus deficient leaf, one finds crossover where trends completely reversed changing wavelength. As result, success involving measurement chlorophyll greatly appropriate selection Fluorescence sensing systems based above are being proposed or developed ground mobile vans, helicopters, small aircraft. goals efforts were better define origins improve our understanding relationship physiological status plant.© (1997) COPYRIGHT SPIE--The International Society Optical Engineering. Downloading abstract is permitted personal only.