Alendronate-Anionic Clay Nanohybrid for Enhanced Osteogenic Proliferation and Differentiation.

摘要: Background Alendronate (AL), a drug for inhibiting osteoclast-mediated bone-resorption, was intercalated into an inorganic delivery nanovehicle, layered double hydroxide (LDH), to form new nanohybrid, AL-LDH, with 1:1 heterostructure along the crystallographic C-axis. Based on intercalation reaction strategy, present AL-LDH system (DDS) realized enhanced efficacy of AL, which confirmed by improved proliferation and osteogenic differentiation osteoblast-like cells (MG63). Methods The nanohybrid synthesized conventional ion-exchange characterized powder X-ray diffraction (PXRD), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FT-IR) spectroscopy. Additionally, in vitro tests, such as cell alkaline phosphatase (ALP) activity, were analyzed. Results AL successfully LDH via reaction, thus prepared DDS single phasic chemically well defined. accumulated content MG63 treated nanoparticles determined be 10.6-fold higher than that within those intact after incubation 1 hour, suggesting intercellular permeation facilitated thanks hybridization vehicle, LDH. Furthermore, both level ALP activity hybrid drug, found much more AL. This is surely due fact could deliver very efficiently, although itself does not show any effect cells. Conclusion considered promising improving