摘要: In this work actin is used to illustrate connection of protein fluorescence characteristics with its structure. On one hand, it has been demonstrated what kind information about the contribution each tryptophan residues bulk spectrum can be obtained from special analysis three-dimensional other potentials intrinsic for elucidation proteins structure, dynamics and processes folding-unfolding are shown. particular, using method a new essentially unfolded kinetic intermediate state was detected characterized, place inactivated predecessor in process determined. It revealed that not between native completely states, as accepted before, but result misfolding. basis data model pathway proposed.
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