Whole-Body Imaging of Hematopoietic and Cancer Cells Using Near-Infrared Probes

摘要: Noninvasive in vivo monitoring of cell behavior presents a major challenge to imaging researchers. Cells labeled with fluorescent probes or expressing bioluminescent reporter genes offer opportunities for tracking. However, it is important verify that labeling remains stable once the cell, does not impair phenotype influence engrafting behavior. Herein, we provide brief outline current approaches whole-body fluorescence cancer and hematopoietic cells by means near-infrared (NIR) fluorescence-emitting agents. Cell-labeling will be reviewed, particular focus on use NIR lipophilic membrane dyes, nontargeted cyanine-based markers, esterase-activatable probes, Quantum Dots. Each approach features both advantages limitations, typically related ease use, sensitivity, specificity, toxicity stability. The beneficial aspects are maximized when used conjunction complementary modalities. Thus, final section this chapter devoted context multimodal imaging.