Construction of a Prognostic Immune-Related LncRNA Risk Model for Lung Adenocarcinoma.

摘要: Background Lung adenocarcinoma (LUAD) originates mainly from the mucous epithelium and glandular of bronchi. It is most common pathologic subtype non-small cell lung cancer (NSCLC). At present, there still a lack clear criteria to predict efficacy immunotherapy. The 5-year survival rate for LUAD patients remains low. Methods All data were downloaded Cancer Genome Atlas (TCGA) database. We used Gene Set Enrichment Analysis (GSEA) database obtain immune-related mRNAs. Immune-related lncRNAs acquired by using correlation test genes with R version 3.6.3 (Pearson coefficient cor = 0.5, P < 0.05). TCGA-LUAD dataset was divided into testing set training randomly. Based on perform univariate multivariate Cox regression analyses, we screened prognostic given risk score each sample. Samples high-risk group low-risk according median score. By combination Kaplan-Meier (KM) curve, receiver operating characteristic (ROC) (AUC) independent factor analysis, clinical samples, assessed accuracy model. Ontology (GO) enrichment analysis Kyoto Encyclopedia Genes Genomes (KEGG) performed differentially expressed mRNAs between group. related immune response two groups analyzed evaluate role model in predicting effects In order explain internal mechanism immunotherapy, epithelial-mesenchymal transition (EMT) groups. extracted RNA normal bronchial epithelial cells verified expression level quantitative real-time polymerase chain reaction (qRT-PCR) test. compared our other published signatures an cohort. transfected siRNA-LINC0253. Western blot observed change EMT-related marker protein level. Results Through 24 found be strongly associated dataset. Utilizing 10 selected establish K-M curves ROC proved that has fine predictive effect. GO indicated effect involved intercellular interaction. KEGG are endocytosis MAPK signaling pathway. immunotherapy significantly different A qRT-PCR AUC 5 years validation showed this had superior accuracy. Conclusion lncRNA established us could better prognosis LUAD.