A model system for analyzing intercellular communication through plasmodesmata using moss protonemata and leaves

摘要: Plant growth, development, and environmental responses require the proper regulation of intercellular movement signals nutrients. For this, plants have specialized cytoplasmic channels, plasmodesmata (PD), which allow symplasmic micro- macromolecules between neighboring cells. Internal external spatio-temporally regulate molecules through PD to control plant development responses. Although some aspects targeted been revealed, mechanisms non-targeted, diffusible flow PD, its function, remain poorly understood, particularly at cellular level. Previously, we developed a system quantitatively analyze non-targeted photoconvertible fluorescent protein, Dendra2, single-cell level in filamentous protonemata tissue moss Physcomitrella patens. In protonemata, one-dimensional communication can be easily observed analyzed this review, describe how leaves P. patens used study discuss help improve our understanding function plants.