摘要: Techniques for the isolation and culture of human mammary epithelial cells are described. The procedure consists dissection followed by partial enzymatic digestion with collagenase hyal-uronidase subsequent filtration to separate clusters from digested stromal elements. Culture procedures utilizing two different growth media presented. A serum-free medium, MCDB170, permits long-term (45 60 population doublings) a pure population; less defined MM, yields fewer doublings but increased expression some mammary-specific properties.
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