Agrobacterium-mediated viral vector-amplified transient gene expression in Nicotiana glutinosa plant tissue culture.

摘要: A viral vector based on the bean yellow dwarf virus was investigated for its potential to increase transient gene expression. An intron-containing GUS reporter and cis-acting regulatory elements were incorporated in could be complemented by replication-associated proteins provided a secondary vector. All vectors delivered Nicotiana glutinosa plant cell suspension or hairy root cultures via auxotrophic Agrobacterium tumefaciens. Cell culture generated greater yield of expression than did culture, as result limitation imposed roots express protein only surface tissue containing actively dividing cells. Reporter increased when construct co-delivered with supplying both replication associated (REP REPA); decreased REP co-delivered. from 0.091% alone 0.22% total soluble (% TSP) Rep-supplying construct. 3 days after initiation bacterial co-culture, providing rapid generation heterologous culture.