Reading two bases twice: mammalian antizyme frameshifting in yeast.

摘要: Abstract Programmed translational frameshifting is essential for the expression of mammalian ornithine decarboxylase antizyme, a protein involved in regulation intracellular polyamines. A cassette containing antizyme frameshift signals found to direct high-level (16%) yeast, Saccharomyces cerevisiae. In contrast +1 system, yeast same frame reached by -2 frameshifting. Two bases are read twice. The likely be mediated slippage mRNA and re-pairing with tRNA P-site. downstream pseudoknot stimulates 30-fold compared 2.5-fold reticulocyte lysates. When length spacer between shift site extended three nucleotides, become equal.