Mycoplasma gallisepticum species and strain-specific recombinant DNA probes.
作者： M.I. Khan , B.C. Kirkpatrick , R. Yamamoto
DOI: 10.1080/03079458908418586
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摘要: Summary Genomic libraries of vaccine (F‐K810) and wild type (S6) Mycoplasma gallisepticum were constructed in Escherichia coli (strain JM83) using the plasmid vector pUC8. Recombinant clones screened by colony, dot Southern hybridisations 32P‐labelled genomic DNA from M. strains K810 S6. Eight identified which contained sequences specific to one clone was a fragment unique strain gallisepticum. When labelled used as probe hybridisation assays, species‐specific recombinant plasmids differentiated standard reference cultures, atypical isolates other avian species. In similar containing strain‐specific several probes provided sens...
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