AtSNX1 defines an endosome for auxin-carrier trafficking in Arabidopsis

摘要: Growth and development of plants are dependent on the levels distribution phytohormone auxin. Auxin trafficking in plant cells is highly regulated by a variety plasma membrane auxin carriers. Jaillais et al. now report discovery new intracellular compartment, SNX1 endosomal which involved one these Trafficking throughout This study reports Polarized cellular its carriers essential for normal growth development. Polar transport 1,2 maintained network influx (AUX)3 efflux (PIN)1,2,4,5,6 Both PIN protein cycling between endosomes require activity GNOM1,2,7,8; however, routes taken remain largely unknown. Here we show that Arabidopsis thaliana SORTING NEXIN 1 (AtSNX1) pathway PIN2, but not PIN1 or AUX1, transported through AtSNX1-containing endosomes. We demonstrate snx1-null mutant exhibits multiple auxin-related defects loss function AtSNX1 severely enhances phenotype weak gnom mutant. In root cells, further localizes to an compartment distinct from GNOM-containing endosomes, PIN2 accumulates this after treatment with phosphatidylinositol-3-OH kinase inhibitor wortmannin gravity stimulus. Our data reveal existence novel endocytic sorting