Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model
作者： Woo-Sung Jung , Sei-Myoung Han , Sung-Min Kim , Mi-Eun Kim , Jun-Sik Lee
DOI: 10.1002/CBIN.10306
关键词:
摘要: The thymus is the central lymphoid organ providing a unique and essential microenvironment for T-cell precursor development into mature functionally competent T-lymphocytes. Thus, it important to develop strategies enhancing thymic regeneration from involution induced by variety of clinical treatments conditions. Hepatocyte growth factor (HGF) promotes proliferation in cell types. We have used stem cell-based HGF gene therapy enhance acute involution. HGF-overexpressing human adipose tissue-derived mesenchymal cells (HGF-hATMSCs) were generated liposomal transfection with pMEX expression vector, constructed inserting gene. Significantly increased these was confirmed reverse transcription-polymerase chain reaction an enzyme-linked immunosorbent assay. produced HGF-hATMSCs enhanced mouse epithelial line interleukin-7 vitro. also examined effect on rats Significant increases size weight, as well number thymocytes (especially, early thymocyte progenitors), seen HGF-hATMSCs-treated compared saline-treated control animals. A stimulatory has therefore been shown, highlighting value treating
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