Possible pathogenic role of cationic anti-DNA autoantibodies in the development of nephritis in patients with systemic lupus erythematosus.

摘要: Extensive studies in murine models of lupus nephritis have shown that cationic anti-DNA autoantibodies nephritogenic potential. We investigated whether antibodies IgG class are also produced vivo patients with active nephritis. Antibodies against DNA the sera from SLE were purified by affinity chromatography on DNA-cellulose, followed nonequilibrium pH gradient electrophoresis and SDS-PAGE. The highly prominent electrophoresis-immunoblots Decreased proteinuria after successful treatment prednisolone was associated disappearance circulation. did bind to heparan sulfate, which is a major glycosaminoglycan glomerular basement membrane, much better than neutral antibodies. results suggest may play certain role development Our study demonstrates strong association between presence antibody humans.