Release of microorganisms from soil with respect to transmission electron microscopy viewing and plate counts

摘要: Microorganisms were detached and washed from soil by various procedures involving blending sonication of the in water or pyrophosphate solution, followed successive low-speed, centrifugal-washing separations suspended cells debris. Some these previously used for separating concentrating transmission electron microscopy viewing. Exhaustive applications separated up to 27% platable soil. Based on filterability, either no longer attached particles, very small particles. The fractionating with debris, however, seemed be strongly it other so that they not filterable. Laboratory-grown cultures added sterile non-sterile did attach materials easily recovered even though low-speed centrifugations being used. Electron evidence released concentrated non-inoculated natural soil, remaining suggests small, probably non-platable, tend release more than do size range 0.3 0.5 μm diameter, larger this, including bacterial fungal spores, are difficult separate Plating data heated preparations agreement this spores. results considered relation validity plate counts direct evaluating microbial flora