A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo.
作者： Jasmer Dalal , Pradeep Kumar , R. K. Chandolia , Shikha Pawaria , Rasika Rajendran
DOI: 10.1038/S41598-019-43038-4
关键词:
摘要: The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations (0, 5, 10 20 µM) cryopreserved. We found that did not impair post-thaw kinetics motility but prevented cholesterol efflux, calcium influx, protected CatSper channels from confirmed intracellular level, expression tyrosine phosphorylated proteins (75 80 kDa) CTC (chlortetracycline) assay. Furthermore, antioxidant ability reflected ferric reducing ability, lower production malondialdehyde reactive oxygen species. Also, we demonstrated treated underwent normal capacitation, zona pellucida binding zygote cleavage indicating fertilizing sperm. In conclusion, report a new role in protecting buffalo
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