Regulation of tyrosine phosphorylation of the nicotinic acetylcholine receptor at the rat neuromuscular lunction

摘要: Abstract The nicotinic acetylcholine receptor (AChR) from the electric organ of T. californica is highly phosphorylated on tyrosine residues in vivo. In contrast, phosphorylation AM rat myotube cultures barely detectable. To determine whether this low level AChR muscle cell due to a lack neuronal innervation, we examined diaphragm Immunofluorescent double labeling cryostat sections using antibodies specific for phosphotyrosine or showed direct colocalization with at neuromuscular junction. Using anti-phosphotyrosine antibodies, immunoblots partially purified demonstrated that contains high levels phosphotyrosine. Denervation induced time-dependent decrease AChR, as measured by immunocytochemical and immunoblot techniques. Tyrosine occurred late development junction, between postnatal days 7 14. These studies suggest innervation regulates may play an important role developmental regulation AChR.