Expression of Putative Fatty Acid Transporter Genes Are Regulated by Peroxisome Proliferator-activated Receptor α and γ Activators in a Tissue- and Inducer-specific Manner

摘要: Regulation of gene expression three putative long-chain fatty acid transport proteins, translocase (FAT), mitochondrial aspartate aminotransferase (mAspAT), and protein (FATP), by drugs that activate peroxisome proliferator-activated receptor (PPAR) alpha gamma were studied using normal obese mice rat hepatoma cells. FAT mRNA was induced in liver intestine cells to various extents only PPARalpha-activating drugs. FATP similarly liver, but a lesser extent intestine. The induction time course the slower for than an encoding peroxisomal enzyme. An obligatory role PPARalpha hepatic demonstrated, since increase these mRNAs not observed PPARalpha-null mice. Levels mAspAT higher treated with proliferators, while levels similar regardless treatment. In white adipose tissue KKAy mice, thiazolidinedione PPARgamma activators (pioglitazone troglitazone) more efficiently activator, clofibrate. This effect absent brown tissue. Under same conditions, did change significantly tissues. conclusion, tissue-specific genes involves both -gamma. Our data suggest among transporters, appear have physiological roles. Thus, proliferators influence metabolism intracellular acids also cellular uptake, which is likely be important regulatory step lipid homeostasis.