An In Vitro Study of Aerosolized Surfactant Carrier Deposition and Dispersion on Airway Surface Models

摘要: Inhaled antibiotics are frequently used for treating infections associated with cystic fibrosis (CF) and under development other uses including hospital-acquired pneumonias. However, the long-term efficacy of inhaled anti-infectives depends largely on uniformity pulmonary drug deposition, which is variable in diseased lungs. In surfactant replacement therapy (SRT), premature infants who lack adequate levels receive a bolus exogenous through an endotracheal tube. The success this due to dispersion from convective flows generated by surface tension gradients along airway surface. Based same mechanism, aerosolized carriers have been proposed as potential means augmenting distribution little experimental evidence exists support this. goal study was assess improve medications lungs following aerosol deposition. This included design delivery system that produced respirable size delivered onto two realistic vitro models first model incorporated porcine gastric mucin (PGM) second utilized human bronchial epithelial (HBE) cell cultures, CF non-CF cells. Differences various surfactants (cationic, anionic, non-ionic) vs. saline (control) were quantified using fluorescence microscopy different sized fluorescent tags acting analogs. spanned range molecular level 1.0 micron polystyrene sphere. On PGM model, enhanced 2-20 fold fairly uniform all tags. When sufficiently hydrated, HBE both non-CF, also demonstrated significant spreading compared saline, similar areas patterns demonstrates Further studies required demonstrate their these vivo applications.