Genotypic Stability of Cold-Adapted Influenza Virus Vaccine in an Efficacy Clinical Trial
作者： Tai-An Cha , Kevin Kao , Jackie Zhao , Patricia E. Fast , Paul M. Mendelman
DOI: 10.1128/JCM.38.2.839-845.2000
关键词:
摘要: An investigational live influenza virus vaccine, FluMist, contains three cold-adapted H1N1, H3N2, and B viruses. The vaccine viruses are 6/2 reassortants, in which the hemagglutinin (HA) neuraminidase (NA) genes derived from circulating wild-type remaining six master donor strains. strains ensure attenuation, HA NA confer ability to induce protective immunity against contemporary genotypic stability of this was studied by employing clinical samples collected during an efficacy trial. Viruses present nasal throat swab specimens supernatants after culturing were detected subtyped multiplex reverse transcriptase (RT)-PCR. Complete genotypes these determined a combination RT-PCR restriction fragment length polymorphism, fluorescent single-strand conformation nucleic acid sequencing analysis. FluMist appeared be genotypically stable replication human host. All 2-week postvaccination period shed had maintained genotype.
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