Definition of a Commonly Deleted Region in Ovarian Cancers to a 300-kb Segment of Chromosome 6q27
作者： Susumu Saito , Kiichiro Noda , Junko Saito , Yusuke Nakamura , Satoru Sagae
DOI:
关键词:
摘要: Allelic deletions of chromosome 6q that occur frequently in ovarian cancers imply the presence a putative tumor suppressor gene this chromosomal vicinity. We analyzed DNA from 32 patients with carcinomas for loss heterozygosity at loci on distal portion and constructed detailed deletion map. The map indicated commonly deleted region between D6S149 (defined by CI6-24) A2, which are estimated to be 300 kb apart basis our cosmid contig By means exon trapping, we found human AF-6 gene, is disrupted acute myeloid leukemia cells carry (6;11)(q27;q23) translocation, located within region. Subsequent screening revealed no mutations. However, mapping results, narrowed containing 300-kb segment 6q27, will facilitate further efforts identify associated cancer.
aacrjournals.org参考文章(12)
Takashi Tokino, Akira Tanigami, Yusuke Nakamura, Masaki Mori, Thomas M Glaser, Thomas M Glaser, J. W. Park, Ei Ichi Takahashi, Carol Jones, Tada Aki Hori, Isolation and mapping of 62 new RFLP markers on human chromosome 11. American Journal of Human Genetics. ,vol. 48, pp. 258- 268 ,(1991)
T Tokino, K Okui, M Oshimura, Y Nakamura, S Saito, Isolation and mapping of 68 RFLP markers on human chromosome 6. American Journal of Human Genetics. ,vol. 50, pp. 65- 70 ,(1992)
Satoru Sagae, Ryuichi Kudo, Sumiko Koi, Kiichiro Noda, Hiroko Saito, Junko Saito, Yusuke Nakamura, Susumu Saito, Fine-Scale Deletion Mapping of the Distal Long Arm of Chromosome 6 in 70 Human Ovarian Cancers Cancer Research. ,vol. 52, pp. 5815- 5817 ,(1992)
E. D. Green, M. V. Olson, Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 1213- 1217 ,(1990) , 10.1073/PNAS.87.3.1213
Takashi Imai, Maynard V. Olson, Second-generation approach to the construction of yeast artificial-chromosome libraries Genomics. ,vol. 8, pp. 297- 303 ,(1990) , 10.1016/0888-7543(90)90285-3
WD Foulkes, J Ragoussis, GWH Stamp, GJ Allan, J Trowsdale, Frequent loss of heterozygosity on chromosome 6 in human ovarian carcinoma. British Journal of Cancer. ,vol. 67, pp. 551- 559 ,(1993) , 10.1038/BJC.1993.101
Y Nakamura, M Leppert, P O'Connell, R Wolff, T Holm, M Culver, C Martin, E Fujimoto, M Hoff, E Kumlin, al. et, Variable number of tandem repeat (VNTR) markers for human gene mapping Science. ,vol. 235, pp. 1616- 1622 ,(1987) , 10.1126/SCIENCE.3029872
Vaskar Saha, Debra M. Lillington, Andrew N. Shelling, Tracy Chaplin, Marie-Laure Yaspo, Trivadi S. Ganesan, Bryan D. Young, AF6 gene on chromosome band 6q27 maps distal to the minimal region of deletion in epithelial ovarian cancer. Genes, Chromosomes and Cancer. ,vol. 14, pp. 220- 222 ,(1995) , 10.1002/GCC.2870140311
Fujio Kasumi, Futoshi Akiyama, Goi Sakamoto, Takaaki Sato, Kazuhiro Yamakawa, Akira Tanigami, Yusuke Nakamura, Allelotype of Breast Cancer: Cumulative Allele Losses Promote Tumor Progression in Primary Breast Cancer Cancer Research. ,vol. 50, pp. 7184- 7189 ,(1990)
Ross S. Berkowitz, Samuel C. Mok, William R. Welch, Debra A. Bell, Gayle Blanchard, Kerry J. Rodabaugh, Detailed Deletion Mapping of Chromosome 6q in Borderline Epithelial Ovarian Tumors Cancer Research. ,vol. 55, pp. 2169- 2172 ,(1995)