Attenuation of oxidative damage to DNA by taurine and taurine analogs.

摘要: Taurine has been suggested to have cytoprotective actions via a number of different mechanisms. The role taurine in protecting DNA from oxidative damage received only limited attention. aim the present studies was test hypothesis that might act attenuate caused by free radicals generated iron-stimulated catecholamine oxidation presence H2O2. Calf thymus (100 μg/tube) exposed reaction mixture containing: ferric chloride (60 μM), H2O2 (2.8 mM) and L-dopa μM).Taurine analogs were added simultaneously determine their effects prevent DNA. carried out for 1 hour at 37° C terminated rapid freezing an ethanol/dry ice bath. precipitated with ethanol subsequently hydrolyzed formic acid under vacuum. hydroxylated bases separated HPLC detected electrochemically. All experiments replicated minimum 5 times. (20 found reduce (p〈0.05) as indexed reductions formation 5-OH-uracil (49%↓)8-OH adenine (37%↓)and 8-OH guanine (21%↓)Taurine had minimal 5-OH cytosine (〈7%↓) also increased total recovery after 3640% undamaged -32%. Uracil could be reduced mM 8-OH-adenine taurine. Studies conducted various amino base adduct 20 β-alanine (30%↓) lysine (58%↓) glutathione (88%↓) When tested mM, both hypotaurine homotaurine provided greater protection against than taurine, whereas isethionic similar level Using identical conditions assays hydroxylation, we whether inhibition quinone account taurine's mechanism action. (49%↓) (24%↓) (79%↓) all formation. Thus, part taurines action inhibit damage, but it not homotaurine's efficacy preventing damage. Overall, these show concentrations normally cells can