Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid.

摘要: The aim of the present work was to assemble extracellular matrix components into polyelectrolyte multilayers using layer-by-layer deposition method. films are constructed with type-I collagen and hyaluronic acid. construction exhibits general features observed during multilayer buildup: alternate positive negative values zeta potential film its regular increase thickness number, n, step. This is shown be linear n. As expected for a linearly growing film, confocal microscopy shows that when brought in contact solution, does not diffuse but interacts only outer layer. However, constituted homogeneously distributed polyanion/polycation complexes as it usually observed, they formed fibers imaged by AFM. typical width these increases number steps. Finally, found chondrosarcoma cells spread well synthesize on ending films, whereas no cellular HA ones. Such architectures may further functionalized inclusion active drugs, peptides, proteins..., could used tunable biomaterial interfaces.