Assessment of the Presence and the Level of BCR-ABL Fusion Gene Expression and Mutational Status in ABL Kinase Domain

摘要: In this chapter, selected molecular methods currently widely applied for the diagnostics and assessment of minimal residual disease (MRD) in patients with chronic myelogenous leukemia (CML) are presented. At time diagnosis, qualitative RT-PCR method is employed. It allows identification type BCR-ABL fusion gene. During follow-up, quantitative RQ-PCR analysis used monitoring kinetics changes gene expression level. The results these analyses might indicate need therapy modification. case undetectable levels RQ-PCR, a more sensitive approach, nested RT-PCR, be performed. lack or loss response, mutational status ABL kinase domain required.