Growth regulation of the B lymphoma cell line WEHI-231 by anti-immunoglobulin, lipopolysaccharide, and other bacterial products.

摘要: The growth of WEHI-231, a murine immature B lymphoma cell line, was inhibited by anti-IgM antibodies. inhibition proliferation, as measured [3H]thymidine incorporation, occurred between 16 and 28 hr after addition anti-IgM. Moreover, the arrest irreversible: cells that were cultured with for 18 then recultured without it failed to recover ability proliferate, even though treated up 30 remained viable, trypan blue exclusion. Three polyclonal activators obtained from bacteria, lipopolysaccharide (LPS), peptidoglycan Staphylococcus aureus, gliding bacterial adjuvant Cytophaga (GBA), able protect WEHI-231 anti-IgM-induced arrest. protection transient, ending approximately 56 hr. This transience shown be due desensitization products. Interestingly, pretreatment any products desensitized all heterologous nature this suggests three these may act through common signaling pathway despite their diverse chemical natures.