Anti-tumor effects of Atractylenolide I on bladder cancer cells

摘要: Atractylenolide I (ATR-1), an active component of Rhizoma Atractylodis Macrocephalae, possesses cytotoxicity against various carcinomas. However, little is known about the effects ATR-1on bladder cancer. In present study, anti-tumor activity ATR-1 was examined on cancer cells both in vivo and vitro. MTT assay used to assess cytotoxic effect ATR-1. Cell cycle distribution apoptosis levels were evaluated using flow cytometry. Western blotting applied measure proteins associated with apoptotic pathway, cell progression PI3K/Akt/mTOR signaling pathway. Tumor models nude mice induced by injection T-24 253J human cells. inhibited proliferation, arrested G2/M phase through up-regulation p21 down-regulation cyclin B1, CDK1 Cdc25c. Meanwhile, also triggered cellular depending activation mitochondrial Mechanism investigation indicated that exerts its relies inhibition Finally, studies showed blocked or 253J-induced xenograft tumor growth without noticeable toxicity. may be served as a potential therapeutic agent for treatment