Current trends in molecular recognition and bioseparation

摘要: Molecular recognition guides the selective interaction of macromolecules with each other in essentially all biological processes. Perhaps most impactful use biomolecular separation science has been affinity chromatography. The results last 26 years, since Cuatrecases, Wilchek and Anfinsen first reported purification staphylococcal nuclease, have validated power specificity for purification. This stimulated an explosion solid-phase ligand designs chromatographic applications. An ongoing case point is recombinant proteins, which aided by engineering proteins to contain Affinity-Tag sequences, such as hexa-histidine metal-chelate epitope sequence immobilized monoclonal antibody. Tag technology can be adapted plate assays techniques. advance chromatography also ligand-based methods characterize macromolecular recognition, including both optical biosensor methods. And, new phage display diversity library approaches continue emerge identify molecules potential ligands. Overall, it tantalizing envision a continued evolution technologies selectivity built into vehicle purification, analysis, screening applications sciences.