A Possible Role of Crustacean Cardioactive Peptide in Regulating Immune Response in Hepatopancreas of Mud Crab.

摘要: Crustacean cardioactive peptide (CCAP), a cyclic amidated non-apeptide, is widely found in arthropods. The functions of CCAP have been revealed to include regulation heart rate, intestinal peristalsis, molting, and osmotic pressure. However, date, there has not any report on the possible involvement immunoregulation crustaceans. In this study, precursor (designated as Sp-CCAP) was identified commercially important mud crab Scylla paramamosain, which could be processed into four CCAP-associated peptides one mature (PFCNAFTGC-NH2). Bioinformatics analysis indicated that Sp-CCAP highly conserved RT-PCR results expressed nerve tissues gonads, whereas receptor gene (Sp-CCAPR) 12 S. including hepatopancreas. situ hybridization further showed an Sp-CCAPR-positive signal mainly localized F-cells Moreover, mRNA expression level Sp-CCAPR hepatopancreas significantly up-regulated after lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid [Poly (I:C)] challenge. Meanwhile, Sp-CCAPR, nuclear transcription factor NF-kappa B homologs (Sp-Dorsal Sp-Relish), member mitogen-activated protein kinase (MAPK) signaling pathway (Sp-P38), pro-inflammatory cytokines (Sp-TNFSF Sp-IL16), antimicrobial (Sp-Lysozyme, Sp-ALF, Sp-ALF4, Sp-ALF5) were all administration synthetic both vivo vitro. addition vitro also led increase nitric oxide (NO) concentration improved bacterial clearance ability culture medium. present study suggested system might involved immune responses paramamosain by activating molecules Collectively, our findings shed new light neuroendocrine-immune regulatory arthropods potentially provide strategy for disease prevention control aquaculture.