High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, Forest Soil, and Sphagnum Moss in Cranesville Swamp Preserve, West Virginia, USA.

摘要: Northern temperate forest soils and Sphagnum-dominated peatlands are a major source sink of methane. In these ecosystems, methane is mainly oxidized by aerobic methanotrophic bacteria, which typically found in aerated soils, surface peat, Sphagnum moss. We contrasted bacterial diversity abundances from the (i) organic horizon soil; (ii) peat; (iii) submerged moss Cranesville Swamp Preserve, West Virginia, using multiplex sequencing 16S rRNA (V3 region) gene amplicons. From ~1 million reads, >50,000 unique OTUs (Operational Taxonomic Units), 29 34 sequences were detected Methylococcaceae Methylocystaceae, respectively, 24 potential methanotrophs Beijerinckiaceae also identified. Methylacidiphilum-like not detected. Proteobacterial bacteria constitute <2% microbiota environments, with Methylocystaceae one to two orders magnitude more abundant than all environments sampled. The less diverse soil compared other habitats. Nonmetric multidimensional scaling analyses indicated that majority tend occur habitat only (peat or moss) co-occurred both peat. This study provides insights into structure communities relationship type, suggests peat can influence methanotroph community biogeography.