High Proportion of Indeterminate QuantiFERON-TB Gold In-Tube Results in an Inpatient Population Is Related to Host Factors and Preanalytical Steps.

摘要: QuantiFERON-TB Gold In-Tube test (QFT-GIT) is an interferon gamma (IFN-γ) release assay (IGRA) that uses enzyme-linked immunosorbent to measure the amount of IFN-γ produced in response vitro stimulation whole blood with specific Mycobacterium tuberculosis (MTB) antigens [1]. IGRAs and tuberculin skin tests (TSTs) are often utilized inpatients either for evaluating latent (TB) infection (LTBI) among high-risk groups, or as adjunctive addition mycobacterial smears/cultures diagnosis active TB. offer several advantages over TST. The logistical advantage a single visit particularly important inpatient setting where patients discharged from hospital transferred off floor before TST can be read. Objective output potential higher specificity BCG-vaccinated individuals additional benefits [2, 3]. Challenges associated include poor reproducibility, variable sensitivity [1], high rates indeterminate results certain groups [4]. Indeterminate due failure positive control tube, low production phytohemagglutinin negative detection levels absence TB [5]. first scenario has been related host immunosuppression [4, 6, 7], preanalytical steps such delayed incubation inadequate shaking [8, 9], manufacturing defects. Elucidating whether result processing factors, optimizing implementation QFT-GIT this setting. Therefore, purpose study was (1) examine proportion hospitalized patients, (2) determine factors results, (3) evaluate changes MTB testing after implementation.