Economic considerations important in the scale-up of an ovalbumin separation from hen egg-white on the anion-exchange cellulose DE92

摘要: The scale-up of the separation hen egg-white proteins has been investigated using Whatman DE92 anion-exchange cellulose. Having developed suitable chromatographic conditions, a maximum binding capacity 100 mg protein/ml packed was determined in 25-ml column. process scaled up 1000-fold and influence batch column techniques on step assessed. Data indicate processes to be more efficient than adsorptive stage.